First Analysis of a Bacterial Collagen-Binding Protein with Collagen Toolkits: Promiscuous Binding of YadA to Collagens May Explain How YadA Interferes with Host Processes
Collagen Type IV
0301 basic medicine
0303 health sciences
Binding Sites
Microscopy, Confocal
Yersinia Infections
Molecular Sequence Data
Bacterial Adhesion
03 medical and health sciences
Platelet Adhesiveness
Peptide Library
Animals
Humans
Cattle
Amino Acid Sequence
Collagen
Adhesins, Bacterial
Yersinia enterocolitica
DOI:
10.1128/iai.01057-09
Publication Date:
2010-05-04T01:09:48Z
AUTHORS (9)
ABSTRACT
ABSTRACT
The
Yersinia
adhesin YadA mediates the adhesion of the human enteropathogen
Yersinia enterocolitica
to collagens and other components of the extracellular matrix. Though YadA has been proposed to bind to a specific site in collagens, the exact binding determinants for YadA in native collagen have not previously been elucidated. We investigated the binding of YadA to collagen Toolkits, which are libraries of triple-helical peptides spanning the sequences of type II and III human collagens. YadA bound to many of them, in particular to peptides rich in hydroxyproline but with few charged residues. We were able to block the binding of YadA to collagen type IV with the triple-helical peptide (Pro-Hyp-Gly)
10
, suggesting that the same site in YadA binds to triple-helical regions in network-forming collagens as well. We showed that a single Gly-Pro-Hyp triplet in a triple-helical peptide was sufficient to support YadA binding, but more than six triplets were required to form a tight YadA binding site. This is significantly longer than the case for eukaryotic collagen-binding proteins. YadA-expressing bacteria bound promiscuously to Toolkit peptides. Promiscuous binding could be advantageous for pathogenicity in
Y. enterocolitica
and, indeed, for other pathogenic bacteria. Many of the tightly binding peptides are also targets for eukaryotic collagen-binding proteins, and YadA was able to inhibit the interaction between selected Toolkit peptides and platelets. This leads to the intriguing possibility that YadA may interfere
in vivo
with host processes mediated by endogenous collagen-binding proteins.
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