First Analysis of a Bacterial Collagen-Binding Protein with Collagen Toolkits: Promiscuous Binding of YadA to Collagens May Explain How YadA Interferes with Host Processes

Collagen Type IV 0301 basic medicine 0303 health sciences Binding Sites Microscopy, Confocal Yersinia Infections Molecular Sequence Data Bacterial Adhesion 03 medical and health sciences Platelet Adhesiveness Peptide Library Animals Humans Cattle Amino Acid Sequence Collagen Adhesins, Bacterial Yersinia enterocolitica
DOI: 10.1128/iai.01057-09 Publication Date: 2010-05-04T01:09:48Z
ABSTRACT
The Yersinia adhesin YadA mediates the adhesion of human enteropathogen enterocolitica to collagens and other components extracellular matrix. Though has been proposed bind a specific site in collagens, exact binding determinants for native collagen have not previously elucidated. We investigated Toolkits, which are libraries triple-helical peptides spanning sequences type II III collagens. bound many them, particular rich hydroxyproline but with few charged residues. were able block IV peptide (Pro-Hyp-Gly)(10), suggesting that same binds regions network-forming as well. showed single Gly-Pro-Hyp triplet was sufficient support binding, more than six triplets required form tight site. This is significantly longer case eukaryotic collagen-binding proteins. YadA-expressing bacteria promiscuously Toolkit peptides. Promiscuous could be advantageous pathogenicity Y. and, indeed, pathogenic bacteria. Many tightly also targets proteins, inhibit interaction between selected platelets. leads intriguing possibility may interfere vivo host processes mediated by endogenous
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