The Multi-Copper-Ion Oxidase CueO of Salmonella enterica Serovar Typhimurium Is Required for Systemic Virulence

• DOI: 10.1128/iai.01208-09
• Publication Date: 2010-03-16T03:54:57Z
• Authors: Maud E. S. Achard; Jai J. Tree; James A. Holden; Kim R. Simpfendorfer; Odilia L. C. Wijburg; Richard A. Strugnell; Mark A. Schembri; Matthew J. Sweet; Michael P. Jennings; Alastair G. McEwan
• Keywords: Salmonella enterica; Multicopper oxidase

ABSTRACT Salmonella enterica serovar Typhimurium possesses a multi-copper-ion oxidase (multicopper oxidase), CueO (also known as CuiD), periplasmic enzyme to be required for resistance copper ions. from S . was expressed recombinant protein in Escherichia coli , and the purified exhibited high cuprous activity. We have characterized an cueO mutant confirmed that it is more sensitive Using murine model of infection, observed significantly attenuated, indicated by reduced recovery bacteria liver spleen, although there no significant difference Peyer's patches mesenteric lymph nodes. However, intracellular survival unprimed or gamma-interferon-primed macrophages not statistically different wild-type suggesting additional host factors are involved clearance mutant. Unlike E. did show greater sensitivity hydrogen peroxide its ions affected siderophores. Similarly, rescued ion toxicity addition branched-chain amino acids leucine.