Chlamydia muridarumT-Cell Antigens Formulated with the Adjuvant DDA/TDB Induce Immunity against Infection That Correlates with a High Frequency of Gamma Interferon (IFN-γ)/Tumor Necrosis Factor Alpha and IFN-γ/Interleukin-17 Double-Positive CD4+T Cells

• DOI: 10.1128/iai.01374-09
• Publication Date: 2010-03-16T03:54:57Z
• Authors: Hong Yu; Xiaozhou Jiang; Caixia Shen; Karuna P. Karunak...; Janina Jiang; Nicole L. Rosin; Robert C. Brunham
• Keywords: CpG Oligodeoxynucleotide

ABSTRACT Major impediments to developing a Chlamydia vaccine lie in identifying immunologically relevant T-cell antigens and delivery manner stimulate protective immunity. Using an immunoproteomic approach, we previously identified three immunodominant (PmpG-1, PmpE/F-2, RplF). Because RplF has high homology human ortholog, it may not be suitable for development. Therefore, this study, evaluated protection against infection the genital tract C57BL/6 mice immunized with -specific membrane proteins PmpG-1, major outer protein (MOMP; as reference) or combination of them formulated one adjuvants, CpG oligodeoxynucleotide (CpG-ODN), AbISCO-100 (AbISCO), DDA/TDB (dimethyldioctadecylammonium bromide/ d -(+)-trehalose 6,6′-dibehenate). The results show that immunization CpG-ODN formulation failed provide infection; AbISCO conferred moderate protection, showed highest degree efficacy. MOMP exhibited greatest among all groups studied. Moreover, also engendered significant BALB/c mice, which have different histocompatibility complex (MHC) background. We measured cell-mediated immune cytokine responses PmpG-1 mixed each adjuvants. demonstrate induced strongest gamma interferon (IFN-γ) interleukin-17 (IL-17) responses, characterized by frequency IFN-γ/tumor necrosis factor alpha (TNF-α) IFN-γ/IL-17 double-positive CD4 + T cells. In conclusion, based on recombinant delivered adjuvant correlated IFN-γ/TNF-α