Inhibition of Staphylococcus epidermidis Biofilm Formation by Rabbit Polyclonal Antibodies against the SesC Protein

• DOI: 10.1128/iai.01464-08
• Publication Date: 2009-06-16T00:49:52Z
• Authors: Mohammad Shahrooei; Vishal Hira; Benoit Stijlemans; Rita Merckx; Peter W. M. Hermans; Johan Van Eldere
• Keywords: Polyclonal antibodies

ABSTRACT Several well-studied proteins with defined roles in Staphylococcus epidermidis biofilm formation are LPXTG motif-containing proteins. Here, we investigate the possible use of protein SesC ( S . surface C; accession no. NP_765787) as a target for antibodies to prevent formation. In vitro and vivo rat model catheter infection, gene expression analysis showed that is expressed more strongly biofilm-associated cells than planktonic particularly during late phase Polyclonal rabbit raised against reduced fibrinogen-binding ability S. RP62A aureus RN4220 transformants expressing SesC, inhibited by strains 10b 1457, significantly numbers bacteria 1-day-old P < 0.001, one-way variance). Our findings revealed promising prevention treatment biofilms because it affects both primary attachment accumulation phases. The precise role remains be identified.