Lipooligosaccharides Containing Phosphorylcholine Delay Pulmonary Clearance of NontypeableHaemophilus influenzae

• DOI: 10.1128/iai.01716-07
• Publication Date: 2008-03-18T00:48:32Z
• Authors: Bing Pang; Dana Winn; Ryan Johnson; Wenzhou Hong; Shayla West-Barnette; Nancy Kock; W. Edward Swords
• Keywords: Phosphorylcholine; Mucociliary clearance

Nontypeable Haemophilus influenzae (NTHi) causes pulmonary infections in patients with chronic obstructive disease and other mucociliary clearance defects. Like many bacteria inhabiting mucosal surfaces, NTHi produces lipooligosaccharide (LOS) endotoxins that lack the O side chain. Persistent populations express a discrete subset of LOS glycoforms, including those containing phosphorylcholine (PCho). In this study, we compared two strains isogenic mutants lacking PCho for from mice following infection. Consistent data model systems, 2019 86-028NP recovered mouse lung contained an increased proportion PCho+ variants to inocula. PCho- were more rapidly cleared. Serial passage both content bacterial resistance clearance, no such increases observed mutants. Increased was also within non-endotoxin-responsive C3H/HeJ Toll-like receptor 4 null (TLR4-/-) mice, albeit at later times postinfection. Changes subpopulations unaffected TLR2-/- parental strain. The occurred earlier time points strain backgrounds all types mice. Comparison tissue cryosections by immunofluorescent staining showed sparse air spaces C57BL/6 large aggregates lungs MyD88-/- These results indicate promotes early infection manner is least partially independent TLR4 pathway.