Identification of Two Translocon Proteins of Vibrio parahaemolyticus Type III Secretion System 2

• DOI: 10.1128/iai.01738-07
• Publication Date: 2008-06-10T00:34:35Z
• Authors: Toshio Kodama; Hirotaka Hiyoshi; Kazuyoshi Gotoh; Yukihiro Akeda; Shigeaki Matsuda; Kwon-Sam Park; Vlademir V. Canta...; Tetsuya Iida; Takeshi Honda
• Keywords: Transport protein

ABSTRACT The type III secretion system (T3SS) translocon complex is composed of several associated proteins, which form a translocation channel through the host cell plasma membrane. These proteins are key molecules that involved in pathogenicity many T3SS-positive bacteria, because they necessary to deliver effector into cells. A T3SS designated T3SS2 Vibrio parahaemolyticus thought be related enterotoxicity this bacterium humans, but mechanism unclear there only one gene (the VPA1362 gene) region homologous other protein genes. It also not known whether functional or it sufficient T3SS2. In study, we identified both (designated VopB2) and VPA1361 VopD2) as T3SS2-dependent proteins. Functional analysis these showed essential for cytotoxicity, (VopT), contact-dependent activity pore formation infected cells vitro. Their targeting membrane depends on T3SS2, furthermore, vivo. results indicate VopB2 VopD2 act V. hence have critical role bacterium.