Phenotypic Differences of Cryptococcus Molecular Types and Their Implications for Virulence in a Drosophila Model of Infection

• DOI: 10.1128/iai.01805-14
• Publication Date: 2014-05-06T03:34:01Z
• Authors: George R. Thompson; Nathaniel Albert; Greg Hodge; Machelle D. Wilson; Jane E. Sykes; Derek J. Bays; Carolina Firacative; Wieland Meyer; Dimitrios P. Kont...
• Keywords: Cryptococcus gattii; Virulence factor; Wild type

ABSTRACT Compared to Cryptococcus neoformans , little is known about the virulence of molecular types in gattii . We compared vitro factor production and survival data using a Drosophila model infection further characterize phenotypic features different cryptococcal types. Forty-nine isolates were inoculated into wild-type flies followed for survival. In assessed growth at 30 37°C, melanin production, capsule size, resistance H 2 O antifungal susceptibility. A mediator was used assess type characteristics as predictors fly model. VGIII most virulent ( P < 0.001). At 30°C, grew rapidly; VNI best. C. capsules larger than those Mediator analysis found strong correlation with 30°C. molecular-type-specific differences temperatures, ability resist hydrogen peroxide, susceptibility, while 30°C strongly associated infection.