Differential Regulation of Proinflammatory Cytokine Expression by Mitogen-Activated Protein Kinases in Macrophages in Response to Intestinal Parasite Infection

• DOI: 10.1128/iai.02279-14
• Publication Date: 2014-08-26T03:35:50Z
• Authors: Mei Xing Lim; Chin Wen Png; Crispina Yan Bing...; Joshua Ding Wei Teo; Huipeng Jiao; Norbert Lehming; Kevin Shyong Wei Tan; Yongliang Zhang
• Keywords: Proinflammatory cytokine

Blastocystis is a common enteric protistan parasite that can cause acute, as well chronic, infection and associated with irritable bowel syndrome (IBS). However, the pathogenic status of remains unclear. In this study, we found antigens induced abundant expression proinflammatory cytokines, including interleukin 1β (IL-1β), IL-6, tumor necrosis factor alpha (TNF-α), in mouse intestinal explants, colitis colon, macrophages. Further investigation utilizing RAW264.7 murine macrophages showed treatment activation ERK, JNK, p38, three major groups mammalian mitogen-activated protein (MAP) kinases play essential roles cytokines. ERK inhibition significantly suppressed both mRNA IL-6 TNF-α IL-1β. On other hand, JNK resulted reductions c-Jun significant suppression all cytokines at levels. Inhibition p38 only no effect on IL-1β TNF-α. Furthermore, serine proteases produced by an important role induction cytokine Our study thus demonstrated for first time could induce various via MAP may contribute to pathogenesis inflammatory diseases through pathways host immune cells, such