Chlamydia psittaci elementary body envelopes: ingestion and inhibition of phagolysosome fusion

• DOI: 10.1128/iai.40.2.741-751.1983
• Publication Date: 2020-01-03T02:56:04Z
• Authors: L G Eissenberg; P B Wyrick; C H Davis; J W Rumpp
• Keywords: Chlamydiae; Chlamydia psittaci; Phagolysosome

The cell surface of Chlamydia psittaci seems important for establishing infection since (i) UV-treated elementary bodies (EB) attach to and are ingested by L cells (ii) heat or antibody treatment decreases attachment promotes the fusion chlamydiae-containing phagosomes with lysosomes in macrophages. In studies reported here, [3H]uridine-labeled EB also persisted mouse resident peritoneal macrophages cells, suggesting that phagosome-lysosome is inhibited. We therefore chose investigate ingestion internal fate isolated purified envelopes both nonprofessional professional phagocytic cells. internalized target host as efficiently whole EB. Transmission electron microscopy whose were marked ferritin revealed persistence individual devoid 3-h observation period. contrast, heated 56 degrees C 15 min consistently found ferritin-labeled phagolysosomes early 30 min. As another index persistence, radioisotopically labeled a Bolton-Hunter analog, [3H]N-succinimidyl propionate, their trichloroacetic acid-precipitable material was followed. A third probe, employed detect non-biodegradable antigen, indirect immunofluorescence. Fluorescein-positive antigens brightly visible 7 days when they inoculated untreated maintained penicillin. But treated UV chloramphenicol, all which prevent conversion infectious into metabolically active reticulate bodies, displayed reduced fluorescence 2 appearance fluorescent on surface. This release envelope occurred absence phagolysosome fusion. data add credence belief spontaneous breakdown autolytic enzyme components must occur preparatory bodies.