CD4+ T-cell clones obtained from cattle chronically infected with Fasciola hepatica and specific for adult worm antigen express both unrestricted and Th2 cytokine profiles

• DOI: 10.1128/iai.62.3.818-827.1994
• Publication Date: 2020-01-03T14:11:49Z
• Authors: W C Brown; W C Davis; D A Dobbelaere; A C Rice-Ficht
• Keywords: Hepatica

The well-established importance of helper T (Th)-cell subsets in immunity and immunoregulation many experimental helminth infections prompted a detailed study the cellular immune response against Fasciola hepatica natural bovine host. T-cell lines established from two cattle infected with F. were characterized for expression surface markers proliferative responses adult worm antigen. Parasite-specific contained mixture CD4+, CD8+, gamma/delta T-cell-receptor-bearing cells. However, cell containing either fewer than 10% CD8+ cells or depleted proliferated vigorously antigen, indicating that these are not required vitro. Seventeen hepatica-specific CD4+ Th-cell clones examined cytokine following concanavalin A stimulation. Biological assays to measure interleukin-2 (IL-2) IL-4, gamma interferon (IFN-gamma), tumor necrosis factor Northern (RNA) blot analysis verify IL-2, IFN-gamma revealed expressed spectrum profiles. Several identified as Th2 by strong IL-4 but little no IL-2 mRNA. majority classified Th0 all three cytokines combinations IFN-gamma. No Th1-cell obtained. All typical memory phenotype, CD45Rlow, lymph node homing receptor (L selectin). These results first describe obtained extend our previous Th1 Babesia bovis (W. C. Brown, V. M. Woods, D. A. E. Dobbelaere, K. S. Logan, Infect. Immun. 61:3273-3281, 1993) include