Interaction of outer envelope proteins of Chlamydia psittaci GPIC with the HeLa cell surface

• DOI: 10.1128/iai.63.9.3600-3608.1995
• Publication Date: 2020-01-06T18:52:19Z
• Authors: L M Ting; R C Hsia; C G Haidaris; P M Bavoil
• Keywords: HeLa; Cell envelope

The chlamydial life cycle involves the intimate interaction of components infectious elementary body (EB) surface with receptors on susceptible eukaryotic cell plasma membrane. We have developed an in vitro ligand binding assay system for identification and characterization detergent-extracted EB envelope proteins capable to glutaraldehyde-fixed HeLa surfaces. With this assay, developmentally regulated cysteine-rich protein Omp2 Chlamydia psittaci strain guinea pig inclusion conjunctivitis was shown bind specifically cells. cells bound selectively over other wall-associated proteins, including major outer membrane protein, abolished under conditions which alter its conformation. Furthermore, trypsin treatment, reduces adherence, resulted proteolytic removal a small terminal peptide at inactivated while having negligible effect protein. Collectively, our results suggest that possesses capacity engage specific host cell. speculate that, since is present only form, observed may be representative determining step pathogenic process.