Purification and characterization of the staphylococcal slime-associated antigen and its occurrence among Staphylococcus epidermis clinical isolates

• DOI: 10.1128/iai.64.8.3410-3415.1996
• Publication Date: 2020-01-06T19:01:16Z
• Authors: L Baldassarri; G Donnelli; A Gelosia; M C Voglino; A W Simpson; G D Christensen
• Keywords: 0301 basic medicine; Antigens, Bacterial; 0303 health sciences; Polysaccharides, Bacterial; Staphylococcal Infections; Ruthenium Red; Acetylglucosamine; Cell Compartmentation; 3. Good health; 03 medical and health sciences; Phenotype; Staphylococcus epidermidis; Humans; Coloring Agents; Microscopy, Immunoelectron

The Staphylococcus epidermidis slime-associated antigen (SAA) was purified and characterized. N-Acetyl-glucosamine accounted for 70% of the dry weight of SAA, which was immunolocalized on the ruthenium red-positive material produced by slime-positive strains. A total of 59% of slime-producing S. epidermidis clinical isolates expressed SAA, while the phenotype slime- SAA+ was never recovered.