Increased Expression of Periplasmic Cu,Zn Superoxide Dismutase Enhances Survival of Escherichia coli Invasive Strains within Nonphagocytic Cells

• DOI: 10.1128/iai.68.1.30-37.2000
• Publication Date: 2009-05-01T17:57:25Z
• Authors: Andrea Battistoni; Francesca Pacello; Silvia Folcarelli; Maria Ajello; Giovanna Donnarumma; Rita Greco; Maria Grazia Amme...; Danièle Touati; Giuseppe Rotilio; Piera Valenti
• Keywords: DNA, Bacterial; 0301 basic medicine; enzyme induction; gene overexpression; Gene Expression; Endosomes; Electron; Plasmid; copper zinc superoxide dismutase; 03 medical and health sciences; Escherichia coli Protein; Escherichia coli Invasive Strains; Periplasmic Cu,Zn Superoxide Dismutase; Nonphagocytic Cells; Escherichia coli; Humans; human; strain difference; endosome; Settore BIO/10 - BIOCHIMICA; bacterium competence; oxidative stre; Microscopy; Base Sequence; Virulence; Superoxide Dismutase; human cell; Escherichia coli Proteins; article; Bacterial; Yersinia pseudotuberculosi; DNA; phagocytosi; Hydrogen-Ion Concentration; 3. Good health; Microscopy, Electron; Oxidative Stress; intracellular killing; priority journal; Genes; Genes, Bacterial; copper zinc superoxide dismutase; article; bacterium competence; endosome; enzyme induction; epithelium cell; Escherichia coli; gene overexpression; HeLa cell; human; human cell; intracellular killing; oxidative stress; phagocytosis; priority journal; strain difference; Yersinia pseudotuberculosis; Base Sequence; DNA, Bacterial; Endosomes; Escherichia coli; Escherichia coli Proteins; Gene Expression; Genes, Bacterial; Hela Cells; Humans; Hydrogen-Ion Concentration; Microscopy, Electron; Oxidative Stress; Plasmids; Superoxide Dismutase; Virulence; epithelium cell; HeLa cell; HeLa Cells; Plasmids

ABSTRACT We have studied the influence of periplasmic Cu,Zn superoxide dismutase on intracellular survival Escherichia coli strains able to invade epithelial cells by expression inv gene from Yersinia pseudotuberculosis but unable multiply intracellularly. Intracellular viability assays, confirmed electron microscopy observations, showed that invasive E. engineered increase production are much more resistant killing than containing only chromosomal sodC copy. However, we found a slight difference in within HeLa between -null mutant and its isogenic wild-type strain. Such small correlates with very low this enzyme also observed acid- oxidative stress-sensitive HB101(pRI203) is rapidly killed GC4468(pRI203). The high mortality HB101(pRI203), independent acidification endosome, abolished overexpression . Our data suggest oxyradicals involved mechanisms bacterial high-level provides bacteria an effective protection against damage. propose could offer important selective advantage host expressing levels enzyme.