Multiple Roles forBordetellaLipopolysaccharide Molecules during Respiratory Tract Infection

• DOI: 10.1128/iai.68.12.6720-6728.2000
• Publication Date: 2002-07-27T10:01:09Z
• Authors: Eric T. Harvill; Andrew Preston; Peggy A. Cotter; Andrew G. Allen; Duncan J. Maskell; Jeff F. Miller
• Keywords: Bordetella bronchiseptica; Respiratory tract

ABSTRACT Bordetella pertussis , parapertussis and bronchiseptica are closely related subspecies that cause respiratory tract infections in humans other mammals express many similar virulence factors. Their lipopolysaccharide (LPS) molecules differ, containing either a complex trisaccharide ( B. ), plus an O-antigen-like repeat or altered ). Deletion of the wlb locus results loss membrane-distal polysaccharide domains three bordetellae, leaving LPS consisting lipid A core oligosaccharide. We have used deletion (Δ ) mutants to investigate roles distal structures infection by bordetellae. Each mutant was defective compared its parent strain colonization tracts BALB/c mice, but location time point at which defects were observed differed significantly. Although Δ much more sensitive complement-mediated killing vitro, they displayed C5 −/− mice with wild-type (wt) indicating increased sensitivity lysis is not sufficient explain vivo defects. Δwlb also wt strains SCID-beige limited interactions adaptive immunity. Interestingly, defective, strain, beginning 1 week postinoculation did differ from ability colonize B-cell- T-cell-deficient suggesting -dependent modifications modulate These data show biosynthesis full-length molecule these bordetellae essential for expression full mice. In addition, indicate different modifying on serve purposes infection, highlighting diversity functions attributable gram-negative bacteria.