Identification of Genes Present Specifically in a Virulent Strain of Klebsiella pneumoniae

DNA, Bacterial 0303 health sciences Base Sequence Virulence Molecular Sequence Data 3. Good health Blotting, Southern Klebsiella pneumoniae 03 medical and health sciences Bacterial Proteins Genes, Bacterial Transcription Factors
DOI: 10.1128/iai.68.12.7149-7151.2000 Publication Date: 2002-07-27T10:01:09Z
ABSTRACT
ABSTRACT Klebsiella pneumoniae is a common cause of septicemia and urinary tract infections. The PCR-supported genomic subtractive hybridization was employed to identify genes specifically present in a virulent strain of K. pneumoniae . Analysis of 25 subtracted DNA clones has revealed 19 distinct nucleotide sequences. Two of the sequences were found to be the genes encoding the transposase of Tn 3926 and a capsule polysaccharide exporting enzyme. Three sequences displayed moderate homology with bvgAS , which encodes a two-component signal transduction system in Bordetella pertussis . The rest of the sequences did not exhibit homology with any known genes. The distribution of these novel sequences varied greatly in K. pneumoniae clinical isolates, reflecting the heterogeneous nature of the K. pneumoniae population.
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