Identification of Genes Present Specifically in a Virulent Strain of Klebsiella pneumoniae
DNA, Bacterial
0303 health sciences
Base Sequence
Virulence
Molecular Sequence Data
3. Good health
Blotting, Southern
Klebsiella pneumoniae
03 medical and health sciences
Bacterial Proteins
Genes, Bacterial
Transcription Factors
DOI:
10.1128/iai.68.12.7149-7151.2000
Publication Date:
2002-07-27T10:01:09Z
AUTHORS (4)
ABSTRACT
ABSTRACT
Klebsiella pneumoniae
is a common cause of septicemia and urinary tract infections. The PCR-supported genomic subtractive hybridization was employed to identify genes specifically present in a virulent strain of
K. pneumoniae
. Analysis of 25 subtracted DNA clones has revealed 19 distinct nucleotide sequences. Two of the sequences were found to be the genes encoding the transposase of Tn
3926
and a capsule polysaccharide exporting enzyme. Three sequences displayed moderate homology with
bvgAS
, which encodes a two-component signal transduction system in
Bordetella pertussis
. The rest of the sequences did not exhibit homology with any known genes. The distribution of these novel sequences varied greatly in
K. pneumoniae
clinical isolates, reflecting the heterogeneous nature of the
K. pneumoniae
population.
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