Biochemical and Immunological Characterization of MP65, a Major Mannoprotein Antigen of the Opportunistic Human Pathogen Candida albicans

• DOI: 10.1128/iai.68.2.694-701.2000
• Publication Date: 2002-07-27T10:03:59Z
• Authors: Maria J. Gomez; Bruno Maras; Alessandra Barca; Roberto La Valle; Donatella Barra; Antonio Cassone
• Keywords: 0301 basic medicine; Antigens, Fungal; Membrane Glycoproteins; Molecular Sequence Data; Lymphocyte Activation; 3. Good health; Fungal Proteins; Mice; 03 medical and health sciences; Candida albicans; Animals; Humans; Amino Acid Sequence

ABSTRACT In the search of antigenic determinants a 65-kDa mannoprotein (MP65) Candida albicans , tryptic fragments immunoaffinity-purified MP65 preparations were tested for their ability to induce lymphoproliferation human peripheral blood mononuclear cells (PBMC). Five major peptides (T1 T5) shown vigorous proliferation PBMC from majority eight healthy subjects tested. With use synthetic peptides, critical amino acid sequences two most immunoactive and T2) determined. Similar what was found molecule, no multiplication induced by in cultures naive cord cells. The sequence analysis chymotryptic demonstrated substantial homology with deduced cell wall proteins Saccharomyces cerevisiae encoded genes YRM305C YGR279C . However, those showing least similarity corresponding regions above proteins. particular, lymphoproliferation-inducing T1 peptide scored only 20% identity homologous S. Besides disclosing MP65, this study provides an initial characterization some its determinants, as well potential detect specific immune responses against target anticandidal cell-mediated immunity humans.