Subunit Vaccination of Mice against New World Cutaneous Leishmaniasis: Comparison of Three Proteins Expressed in Amastigotes and Six Adjuvants

• DOI: 10.1128/iai.68.3.1328-1336.2000
• Publication Date: 2002-07-27T10:01:19Z
• Authors: Toni Aebischer; Markus Wolfram; Silke I. Patzer; Thomas Ilg; Martin Wiese; Peter Overath
• Keywords: Amastigote; Leishmania mexicana

ABSTRACT A mixture of well-defined recombinant antigens together with an adjuvant that preferentially stimulates specific gamma interferon (IFN-γ)-secreting helper type 1 CD4 + T cells (Th1 cells) presents a rational option for vaccine against leishmaniasis. The potential this approach was investigated in murine infections Leishmania mexicana , which are characterized by the absence parasite-specific Th1 response and uncontrolled parasite proliferation. three (glycoprotein 63, cysteine proteinases, membrane-bound acid phosphatase), all expressed amastigotes, mammalian stage parasite, were used immunization C57BL/6 mice combination six adjuvants (interleukin 12 [IL-12], Detox, 4′-monophosphoryl lipid A, QS-21, Mycobacterium bovis BCG, Corynebacterium parvum ). All formulations containing protective challenge promastigotes, insect controlled healed but developed transient and, certain cases, accentuated disease. most effective IL-12 followed Detox. Further studies using these two showed similar effect observed corresponding native proteins, had infection preponderance IFN-γ-secreting lymph nodes draining lesion. Using proteins individually, it is shown relatively abundant proteinases glycoprotein not phosphatase, able to elicit response. results discussed comparison previous subunit vaccines respect cell biological aspects antigen presentation -infected macrophages.