Trehalose 6,6′-Dimycolate (Cord Factor) ofMycobacterium tuberculosisInduces Foreign-Body- and Hypersensitivity-Type Granulomas in Mice

• DOI: 10.1128/iai.69.2.810-815.2001
• Publication Date: 2002-07-27T10:01:19Z
• Authors: Hirokazu Yamagami; Takayuki Matsumoto; Nagatoshi Fujiwara; Tetsuo Arakawa; Kenji Kaneda; Ikuya Yano; Kazuo Kobayashi
• Keywords: Pathogenesis; Proinflammatory cytokine

ABSTRACT Granulomatous inflammation is characterized morphologically by a compact organized collection of macrophages and their derivatives. It classified as either hypersensitivity type or foreign-body type. Lipid components the Mycobacterium tuberculosis cell wall participate in pathogenesis infection. Strains M. have cord factor (trehalose 6,6′-dimycolate [TDM]) on surface. To clarify host responses to TDM, including immunogenicity pathogenicity, we analyzed footpad reaction, histopathology, cytokine profiles experimental granulomatous lesions immunized unimmunized mice challenged with TDM. In present study, demonstrated for first time that TDM can induce both foreign-body-type (nonimmune) hypersensitivity-type (immune) granulomas acting nonspecific irritant T-cell-dependent antigen. Immunized developed more severe than mice. At active lesion, found monocyte chemotactic, proinflammatory, immunoregulatory cytokines. The level was enhanced This result implies nonimmune immune mechanisms induced mycobacterial Taken together previous report, this study shows pleiotropic molecule against plays an important role tuberculosis.