Role ofHelicobacter pylori cagRegion Genes in Colonization and Gastritis in Two Animal Models

• DOI: 10.1128/iai.69.5.2902-2908.2001
• Publication Date: 2002-07-27T10:01:19Z
• Authors: Kathryn A. Eaton; Dange Kersulyte; Megan Mefford; Stephen J. Danon; Steven Krakowka; Douglas E. Berg
• Keywords: Pathogenicity island; Helicobacter

ABSTRACT The Helicobacter pylori chromosomal region known as the cytotoxin-gene associated pathogenicity island ( cag PAI) is with severe disease and encodes proteins that are believed to induce interleukin (IL-8) secretion by cultured epithelial cells. objective of this study was evaluate relationship between PAI, induction IL-8, neutrophilic gastric inflammation. Germ-free neonatal piglets conventional C57BL/6 mice were given wild-type or deficient mutant derivatives H. strain 26695 SS1. Bacterial colonization determined plate count, gastritis inflammation quantified, IL-8 in AGS cells enzyme-linked immunosorbent assay. Deletion entire interruption virB10 virB11 homolog had no effect on bacterial colonization, gastritis, In contrast, these mutations variable effects induction, depending strain. piglet-adapated 26695, which induced cells, deletion PAI decreased induction. mouse-adapted SS1, did not secretion, II any three genes increased These results indicate (i) neither nor ability vitro essential for (ii) there direct presence gastritis.