Direct Involvement of Type II Secretion System in Extracellular Translocation of Shewanella oneidensis Outer Membrane Cytochromes MtrC and OmcA

• DOI: 10.1128/jb.00514-08
• Publication Date: 2008-05-24T00:54:25Z
• Authors: Liang Shi; Shuang Deng; Matthew J. Marshall; Zheming Wang; David W. Kennedy; Alice C. Dohnalkova; Heather M. Mottaz; Eric A. Hill; Yuri A. Gorby; Alexander S. Beliaev; David J. Richardson; John M. Zachara; James K. Fredrickson
• Keywords: 0301 basic medicine; 570; Shewanella; 03 medical and health sciences; Bioresource and Agricultural Engineering; 617; 610; Cytochromes; Membrane Transport Proteins; Gene Deletion; Bacterial Outer Membrane Proteins

ABSTRACT MtrC and OmcA are cell surface-exposed lipoproteins important for reducing solid metal oxides. Deletions of type II secretion system (T2SS) genes reduced their extracellular release and their accessibility to the proteinase K treatment, demonstrating the direct involvement of T2SS in translocation of MtrC and OmcA to the bacterial cell surface.