BdlA, a Chemotaxis Regulator Essential for Biofilm Dispersion in Pseudomonas aeruginosa

• DOI: 10.1128/jb.00599-06
• Publication Date: 2006-10-18T23:23:35Z
• Authors: Ryan Morgan; Steven Kohn; Sung-Hei Hwang; Daniel J. Hassett; Karin Sauer
• Keywords: Swarming motility

ABSTRACT Multiple environmental cues have been shown to trigger biofilm detachment, the transition from surface-attached, highly organized communities known as biofilms motile lifestyle. The goal of this study was identify a gene product involved in sensing that dispersion Pseudomonas aeruginosa . To do so, we focused on novel putative chemotaxis transducer proteins could potentially be sensing. We identified locus encoding such protein played role indicated by observation an isogenic mutant not disperse response variety cues. termed bdlA for b iofilm d ispersion l ocus. BdlA harbors MCP ( m ethyl-accepting c hemotaxis p rotein) domain and two PAS P er- A rnt- S int) domains essential responding signals other proteins. dispersion-deficient phenotype confirmed treatment with biocide H 2 O microscopic observations. independent motility. were found increased adherent properties intracellular levels cyclic di-GMP (c-di-GMP). Our findings suggest may link between cues, c-di-GMP levels, detachment. Based our findings, possible involvement signaling cascade resulting is discussed.