LuxS-Based Quorum Sensing Does Not Affect the Ability ofSalmonella entericaSerovar Typhimurium To Express the SPI-1 Type 3 Secretion System, Induce Membrane Ruffles, or Invade Epithelial Cells

• DOI: 10.1128/jb.00727-09
• Publication Date: 2009-09-26T01:10:52Z
• Authors: Charlotte A. Perrett; Michail H. Karavolos; Suzanne Humphrey; Pietro Mastroeni; Isabel Martinez-A...; Hannah Spencer; David Bulmer; Klaus Winzer; Emma McGhie; Vassilis Koronakis; Paul Williams; C. M. Anjam Khan; Mark A. Jepson
• Keywords: Salmonella enterica; Autoinducer; Wild type

Bacterial species can communicate by producing and sensing small autoinducer molecules a process known as quorum sensing. Salmonella enterica produces 2 (AI-2) via the luxS synthase gene, which is used some bacterial pathogens to coordinate virulence gene expression with population density. We investigated whether might affect ability of serovar Typhimurium invade epithelial cells. No differences were found between wild-type strain S. Typhimurium, SL1344, its isogenic mutant respect number morphology membrane ruffles induced or their The dynamics ruffling also similar in (SL1344) mutant. Furthermore, comparing pathogenicity island 1 (SPI-1) type 3 secretion profiles SL1344 Western blotting measuring single-copy green fluorescent protein fusion prgH (an essential SPI-1 gene) promoter indicated that activity are Genetic deletion did not alter mouse model, therefore, it appears does play significant role regulating invasion vitro vivo.