Activation of Dormant Bacterial Genes byNonomuraeasp. Strain ATCC 39727 Mutant-Type RNA Polymerase

• DOI: 10.1128/jb.01311-08
• Publication Date: 2008-12-02T01:53:59Z
• Authors: Adelfia Talà; Guojun Wang; Martina Zemanova; Susumu Okamoto; Kozo Ochi; Pietro Alifano
• Keywords: Strain (injury)

There is accumulating evidence that the ability of actinomycetes to produce antibiotics and other bioactive secondary metabolites has been underestimated due presence cryptic gene clusters. The activation dormant genes therefore one most important areas experimental research for discovery drugs in these organisms. recent observation several possess two RNA polymerase beta-chain (rpoB) opened up possibility, explored this study, developing a new strategy activate expression bacteria. Two rpoB paralogs, rpoB(S) rpoB(R), provide Nonomuraea sp. strain ATCC 39727 with functionally distinct developmentally regulated polymerases. product which increases after transition stationary phase, characterized by five amino acid substitutions located within or close so-called rifampin resistance clusters play key role fundamental activities polymerase. Here, we report rpoB(R) markedly activated antibiotic biosynthesis wild-type Streptomyces lividans 1326 also KO-421, relaxed (rel) mutant unable ppGpp. Site-directed mutagenesis demonstrated rpoB(R)-specific missense H426N mutation was essential metabolism. Our observations indicated mutant-type duplicated, often exists nature among rare will thus basis further basic applied research.