γ-Butyrolactone-Dependent Expression of theStreptomycesAntibiotic Regulatory Protein GenesrrYPlays a Central Role in the Regulatory Cascade Leading to Lankacidin and Lankamycin Production inStreptomyces rochei

• DOI: 10.1128/jb.01383-07
• Publication Date: 2007-12-15T01:53:31Z
• Authors: Shouji Yamamoto; Yuxi He; Kenji Arakawa; Haruyasu Kinashi
• Keywords: Overproduction

ABSTRACT Our previous studies revealed that the srrX and srrA genes carried on large linear plasmid pSLA2-L constitute a γ-butyrolactone-receptor system in Streptomyces rochei . Extensive transcriptional analysis has now showed antibiotic regulatory protein gene srrY , which is also pSLA2-L, target of receptor/repressor SrrA plays central role lankacidin lankamycin production. The was expressed growth-dependent manner, slightly preceding expression undetectable mutant but restored double mutant. In addition, bound specifically to promoter region this binding prevented by addition S. γ-butyrolactone fraction, while W119A receptor kept even presence γ-butyrolactone. Furthermore, introduction an intact under control foreign into or ( ) All these results confirmed signaling pathway from through leading