Dissection of Ammonium Uptake Systems inCorynebacterium glutamicum: Mechanism of Action and Energetics of AmtA and AmtB

• DOI: 10.1128/jb.01896-07
• Publication Date: 2008-02-02T01:54:54Z
• Authors: Britta Walter; Melanie Küspert; Daniel Ansorge; Reinhard Krämer; Andreas Burkovski
• Keywords: Corynebacterium glutamicum

ABSTRACT Corynebacterium glutamicum has two different Amt-type proteins. While AmtB a low substrate affinity and is not saturable up to 3 mM methylammonium, AmtA high mediates saturable, membrane potential-dependent transport, resulting in steady-state accumulation of even the absence metabolic trapping.