Exoprotein Production Correlates with Morphotype Changes of Nonmotile Shewanella oneidensis Mutants

• DOI: 10.1128/jb.02187-12
• Publication Date: 2013-01-19T05:49:59Z
• Authors: Miaomiao Shi; Lin Wu; Yu Xia; Haijiang Chen; Qixia Luo; Linlin Sun; Haichun Gao
• Keywords: Shewanella oneidensis

We report a previously undescribed mechanism for the rugose morphotype in Shewanella oneidensis, research model investigating redox transformations of environmental contaminants. Bacteria may form smooth or colonies on agar plates. In general, conversion from to colony is attributed increased production exopolysaccharide (EPS). this work, we discovered that aflagellate S. oneidensis mutants grew into colonies, whereas those with nonfunctional flagellar filaments remained smooth. EPS was not altered either case, but showed significantly reduced exoprotein secretion. The idea exoproteins at level correlate rugosity gained support suppressor strains an fliD (encoding capping protein) mutant, which restored levels wild-type and mutant morphotype. Further analyses revealed SO1072 (a putative GlcNAc-binding one highly upregulated these strains. Most intriguingly, study identified compensatory flagellins possibly mediated by bis-(3'-5')-cyclic dimeric GMP.