A locus encoding host range is linked to the common nodulation genes of Bradyrhizobium japonicum

• DOI: 10.1128/jb.169.6.2631-2638.1987
• Publication Date: 2016-11-02T23:22:20Z
• Authors: A J Nieuwkoop; Z Banfalvi; N Deshmane; D Gerhold; M G Schell; K M Sirotkin; G Stacey
• Keywords: Bradyrhizobium japonicum; Rhizobium leguminosarum; Bradyrhizobium

By using cloned Rhizobium meliloti, leguminosarum, and sp. strain MPIK3030 nodulation (nod) genes as hybridization probes, homologous regions were detected in the slow-growing soybean symbiont Bradyrhizobium japonicum USDA 110. These found to cluster within a 25-kilobase (kb) region. Specific nod probes from R. meliloti used identify nodA-, nodB-, nodC-, nodD-like sequences clustered on two adjacent HindIII restriction fragments of 3.9 5.6 kb. A 785-base-pair sequence was identified between nodD nodABC. This contained an open reading frame 420 base pairs oriented same direction specific probe leguminosarum nodIJ-like which also 5.6-kb fragment. hsn (host specificity)-like essential for siratro (Macroptilium atropurpureum) 3.3-kb fragment downstream nodIJ. transposon Tn5 insertion this region prevented siratro, but caused little or no delay (Glycine max).