Nucleotide sequence of the Rickettsia prowazekii citrate synthase gene

• DOI: 10.1128/jb.169.8.3564-3572.1987
• Publication Date: 2016-11-02T23:12:22Z
• Authors: D O Wood; L R Williamson; H H Winkler; D C Krause
• Keywords: Rickettsia prowazekii; Homology

The Rickettsia prowazekii citrate synthase (gltA) gene, previously cloned in Escherichia coli, was localized to a 2.0-kilobase chromosomal fragment. DNA sequence analysis of portion this fragment revealed an open reading frame 1,308 base pairs that encodes protein 435 amino acids with molecular weight 49,171. This translation product is comparable size both the E. coli and pig heart monomers synthesized minicells containing rickettsial gene. Comparisons between deduced acid R. those enzymes extensive homology (59%) two bacterial proteins. In contrast, only 20% enzyme residues were shared functionally similar residues. Upstream from close proximity one another, sequences consensus for RNA polymerase ribosome binding identified. S1 nuclease mapping experiments demonstrated start transcription gene located upstream region. Codon usage gltA found be very biased differed pattern observed coli. Adenine uracil used preferentially third position codons.