p-cresol methylhydroxylase from a denitrifying bacterium involved in anaerobic degradation of p-cresol

• DOI: 10.1128/jb.173.3.1298-1301.1991
• Publication Date: 2016-11-04T18:18:25Z
• Authors: D J Hopper; I D Bossert; M E Rhodes-Roberts
• Keywords: Cresol; Electron acceptor

A bacterium, strain PC-07, previously isolated as part of a coculture capable growing on p-cresol under anaerobic conditions with nitrate the acceptor was identified an Achromobacter sp. The first enzyme pathway, methylhydroxylase, which converts its substrate into p-hydroxybenzyl alcohol, purified. had Mr 130,000 and spectrum flavocytochrome. It composed flavoprotein subunits 54,000 cytochrome 12,500. midpoint redox potential 232 mV. Km kcat for were 21 microM 112 s-1 respectively, phenazine methosulfate, artificial used in assays, determined to be 1.7 mM. These properties place same class methylhydroxylases from aerobically Pseudomonas spp.