Characterization of a mutant strain of Saccharomyces cerevisiae with a deletion of the RAD27 gene, a structural homolog of the RAD2 nucleotide excision repair gene

• DOI: 10.1128/jb.177.2.364-371.1995
• Publication Date: 2016-11-09T13:37:39Z
• Authors: M S Reagan; C Pittenger; W Siede; E C Friedberg
• Keywords: Methane sulfonate; Methyl methanesulfonate

We have constructed a strain of Saccharomyces cerevisiae with deletion the YKL510 open reading frame, which was initially identified in chromosome XI as homolog RAD2 nucleotide excision repair gene (A. Jacquier, P. Legrain, and B. Dujon, Yeast 8:121-132, 1992). The mutant exhibits increased sensitivity to UV light alkylating agent methylmethane sulfonate but not ionizing radiation. renamed frame RAD27 gene, keeping accepted nomenclature for radiation-sensitive yeast mutants. Epistasis analysis indicates that is RAD6 group genes, are involved DNA damage tolerance. also plasmid loss, spontaneous mutagenesis, temperature-sensitive lethality whose phenotype suggests defect replication. Levels transcript cell cycle regulated manner similar those several other genes products known be discuss possible role Rad27 protein