Flagellar filament structure and cell motility of Salmonella typhimurium mutants lacking part of the outer domain of flagellin

• DOI: 10.1128/jb.177.4.1090-1093.1995
• Publication Date: 2016-11-09T16:27:37Z
• Authors: K Yoshioka; S Aizawa; S Yamaguchi
• Keywords: Flagellin

We have isolated spontaneous mutants of Salmonella typhimurium which can swim in the presence antifilament antibodies. The molecular masses flagellins from these were smaller than that (52 kDa) wild-type flagellin. Two produced smallest (42 and 41 selected, domain structures analyzed by trypsin digestion then subjected to amino acid sequencing. two deletions at Ala-204 Lys-292 Thr-183 Lys-279, respectively. These deleted parts belong outer (D3) flagellin, is believed be surface filament. mutant filaments aggregated side salt, resulting disordered motility.