The ars operon of Escherichia coli confers arsenical and antimonial resistance

• DOI: 10.1128/jb.177.4.981-986.1995
• Publication Date: 2016-11-09T16:27:37Z
• Authors: A Carlin; W Shi; S Dey; B P Rosen
• Keywords: Adenosine Triphosphatases; Antimony; 0301 basic medicine; Arsenites; Arsenite Transporting ATPases; Escherichia coli Proteins; Biological Transport; Drug Resistance, Microbial; Gene Expression Regulation, Bacterial; Ion Pumps; Chromosomes, Bacterial; 3. Good health; 03 medical and health sciences; Bacterial Proteins; Genes, Bacterial; Multienzyme Complexes; Operon; Escherichia coli; Arsenates; Cloning, Molecular; Energy Metabolism; Plasmids; Sequence Deletion

The chromosomally encoded arsenical resistance (ars) operon subcloned into a multicopy plasmid was found to confer moderate level of arsenite and antimonite in Escherichia coli. When the deleted from chromosome, cells exhibited hypersensitivity arsenite, antimonite, arsenate. Expression ars genes inducible by arsenite. By Southern hybridization, all strains E. coli examined but not Salmonella typhimurium, Pseudomonas aeruginosa, or Bacillus subtilis.