A vancomycin-inducible lacZ reporter system in Bacillus subtilis: induction by antibiotics that inhibit cell wall synthesis and by lysozyme

• DOI: 10.1128/jb.178.21.6305-6309.1996
• Publication Date: 2016-11-10T13:32:20Z
• Authors: A T Ulijasz; A Grenader; B Weisblum
• Keywords: Enterococcus faecium; Bacitracin; Lysin; Autolysin; Lipid II

We have constructed a Bacillus subtilis strain in which expression of vanH::lacZ gene fusion is regulated by VanR and VanS Enterococcus faecium. This construct allows nonpathogenic bacterial to be used as model system for studying regulation vancomycin resistance. Antibiotics enzymes that affect cell wall biosynthesis stability were tested the ability induce lacZ expression. As result, fosfomycin D-cycloserine added group peptidoglycan synthesis inhibitors shown from vanH promoter. Induction hydrolytic enzymes, well antibiotics whose actions may lead accumulation chemically different precursors, raises possibility models postulate induction [correction peptidodoglycan] precursors are wrong.