Regulation of VanB-type vancomycin resistance gene expression by the VanS(B)-VanR (B) two-component regulatory system in Enterococcus faecalis V583

• DOI: 10.1128/jb.178.5.1302-1309.1996
• Publication Date: 2016-11-10T14:23:09Z
• Authors: S Evers; P Courvalin
• Keywords: Teicoplanin; Gene cluster; Enterococcus faecalis

Acquired VanA- and VanB-type glycopeptide resistance in enterococci is due to synthesis of modified peptidoglycan precursors terminating D-lactate. As opposed VanA-type strains which are resistant both vancomycin teicoplanin, remain teicoplanin susceptible. We have determined the sequence a 7,160-bp DNA fragment associated with Enterococcus faecalis V583 that contains seven open reading frames. The distal part encoded VanH (B), VanB, VanX (B) proteins highly similar putative VanH, VanA, responsible for resistance. Upstream from structural genes these were vanY(B) gene encoding D,D-carboxypeptidase an frame vanW unknown function. proximal cluster coded apparent VanS(B)-VanR two-component regulatory system. VanR was related response regulators OmpR subclass, VanS membrane-associated histidine protein kinases. Analysis transcriptional fusions reporter promoter mapping indicated B-VanS B system activates located immediately downstream vanS gene. Vancomycin, but not inducer, explains susceptibility enterococci.