Increased production of colicin E1 in stationary phase

• DOI: 10.1128/jb.178.7.1928-1935.1996
• Publication Date: 2016-11-14T16:55:50Z
• Authors: J M Eraso; M Chidambaram; G M Weinstock
• Keywords: rpoS; Colicin; Overproduction

The synthesis of colicin E1 is known to be regulated by the SOS response, anaerobiosis, and catabolite repression. expression cea-lacZ fusions was also found stimulated when cells reached stationary phase. This increase in determined due depletion nutrients from medium, since addition fresh medium reversed effect. Expression fusion increased were starved 10 mM MgSO4 they grown conditioned which had been previously. stimulation occurred present single-copy as well multicopy plasmids. Finally, data consistent with this being independent repression, integration host factor stationary-phase regulators encoded rpoS lrp.