Molecular Physiology of Sugar Catabolism in Lactococcus lactis IL1403

• DOI: 10.1128/jb.183.13.3817-3824.2001
• Publication Date: 2002-07-27T10:01:09Z
• Authors: Sergine Even; Nic D. Lindley; Muriel Cocaign-Bo...
• Keywords: Catabolism

The metabolic characteristics of Lactococcus lactis IL1403 were examined on two different growth media with respect to the physiological response sugars, glucose and galactose. Analysis specific rates indicated that despite significant variations in both sugar consumption, homolactic fermentation was maintained for all cultures due low concentration either pyruvate-formate lyase or alcohol dehydrogenase. When ionophore monensin added medium, flux through glycolysis not increased, suggesting a catabolic limitation, which, intracellular concentrations glycolytic intermediates high vivo enzyme capacities, may be at level transport. To assess transcription, novel DNA macroarray technology employed RNA labeled vitro digoxigenin detection hybrids an alkaline phosphatase-antidigoxigenin conjugate. This method showed several genes expressed higher levels mixed-acid pathway synthesis are compared transcript concentrations, it can deduced some translational regulation occurs threefold-higher efficiency cells grown glucose.