High-Salinity-Induced Iron Limitation in Bacillus subtilis

• DOI: 10.1128/jb.184.3.718-727.2002
• Publication Date: 2009-04-23T18:32:58Z
• Authors: Tamara Hoffmann; Alexandra Schütz; Margot Brosius; Andrea Völker; Uwe Völker; Erhard Bremer
• Keywords: Proteome

ABSTRACT Proteome analysis of Bacillus subtilis cells grown at low and high salinity revealed the induction 16 protein spots repression 2 spots, respectively. Most these were identified by mass spectrometry. Four high-salinity-induced proteins corresponded to DhbA, DhbB, DhbC, DhbE, enzymes that are involved in synthesis 2,3-dihydroxybenzoate (DHB) its modification esterification iron siderophore bacillibactin. These encoded dhbACEBF operon, which is negatively controlled central regulatory Fur derepressed upon limitation. We found limitation dhb expression a similar extent both led accumulation comparable amounts DHB culture supernatant. production increased linearly with degree growth medium but could still be reduced an excess iron. Such also partially reversed defect exhibited salt-stressed B. cultures. Taken together, findings strongly suggest experience In support this notion, we several genes operons encoding putative uptake systems was salt stress. The unexpected finding high-salinity stress has component might special ecophysiological importance for natural settings, bioavailable usually scarce.