Molecular Characterization and Transcriptional Analysis of adhE2 , the Gene Encoding the NADH-Dependent Aldehyde/Alcohol Dehydrogenase Responsible for Butanol Production in Alcohologenic Cultures of Clostridium acetobutylicum ATCC 824

• DOI: 10.1128/jb.184.3.821-830.2002
• Publication Date: 2009-04-23T18:32:58Z
• Authors: Lisa Fontaine; Isabelle Meynial-...; Laurence Girbal; Xinghong Yang; Christian Croux; Philippe Soucaille
• Keywords: Clostridium acetobutylicum; Butyraldehyde

ABSTRACT The adhE2 gene of Clostridium acetobutylicum ATCC 824, coding for an aldehyde/alcohol dehydrogenase (AADH), was characterized from molecular and biochemical points view. 2,577-bp codes a 94.4-kDa protein. is expressed, as monocistronic operon, in alcohologenic cultures not solventogenic cultures. Primer extension analysis identified two transcriptional start sites 160 215 bp upstream the codon. expression plasmid DG1 mutant C. , cured pSOL1 megaplasmid, restored butanol production provided elevated activities NADH-dependent butyraldehyde dehydrogenases. recombinant AdhE2 protein expressed E. coli Strep -tag fusion purified to homogeneity also demonstrated activities. This second AADH our knowledge this first example bacterium with AADHs. It noteworthy that corresponding genes, adhE are carried by megaplasmid 824.