clpPofStreptococcus salivariusIs a Novel Member of the Dually Regulated Class of Stress Response Genes in Gram-Positive Bacteria

• DOI: 10.1128/jb.185.2.683-687.2003
• Publication Date: 2003-01-02T19:30:24Z
• Authors: Arnaud Chastanet; Tarek Msadek
• Keywords: 0301 basic medicine; Transcription, Genetic; [SDV]Life Sciences [q-bio]; Molecular Sequence Data; groe; heat-shock; Gram-Positive Bacteria; 03 medical and health sciences; Bacterial Proteins; expression; Humans; Promoter Regions, Genetic; bacillus-subtilis; Heat-Shock Proteins; competence development; Adenosine Triphosphatases; 0303 health sciences; Base Sequence; staphylococcus-aureus; virulence genes; Serine Endopeptidases; Streptococcus; Endopeptidase Clp; Gene Expression Regulation, Bacterial; sequence; 3. Good health; [SDV] Life Sciences [q-bio]; DNA-Binding Proteins; Repressor Proteins; dnak; listeria-monocytogenes; sequence;groe;dnak;expression;virulence genes;competence development;staphylococcus-aureus;listeria-monocytogenes;bacillus-subtilis;heat-shock; Heat-Shock Response

ABSTRACTNucleotide sequence analysis of theStreptococcus salivarius clpPlocus revealed potential binding sites for both the CtsR and HrcA repressors. Dual regulation by HrcA and CtsR was demonstrated by usingBacillus subtilisas a heterologous host, and CtsR was shown to bind directly to theclpPpromoter sequence. This is the first example of aclpPgene under the control of HrcA.