Transcription Regulation by the Mycobacterium tuberculosis Alternative Sigma Factor SigD and Its Role in Virulence

• DOI: 10.1128/jb.186.19.6605-6616.2004
• Publication Date: 2004-09-16T18:43:10Z
• Authors: Sahadevan Raman; Rohan Hazra; Christopher C. Da...; Robert N. Husson
• Keywords: Sigma factor; Virulence factor

ABSTRACT Mycobacterium tuberculosis , an obligate mammalian pathogen, adapts to its host during the course of infection via regulation gene expression. Of regulators transcription that play a role in this response, several alternative sigma factors M. have been shown control expression response stresses, and some these are required for virulence or persistence vivo. For study, we examined factor SigD virulence. Using microarray analysis, identified genes whose was altered strain with sigD deletion. A small number genes, including itself, encoding autocrine growth RpfC, unknown function, Rv1815, appear be directly regulated by factor. By identifying vivo promoters determined consensus promoter sequence is putatively recognized SigD. The PE-PGRS proteins, part large family related significantly increased mutant. We found stable throughout log phase stationary but it declines rapidly oxygen depletion. In mouse model, mutant attenuated, differences survival inflammatory lung between mice infected those wild type.