PriA Is Essential for Viability of the Escherichia coli Topoisomerase IV parE10 (Ts) Mutant

• DOI: 10.1128/jb.186.4.1197-1199.2004
• Publication Date: 2004-02-04T23:24:31Z
• Authors: Gianfranco Grompone; Vladimir Bidnenko; S. Dusko Ehrlich; Bénédicte Michel
• Keywords: Adenosine Triphosphatases; DNA Replication; DNA Topoisomerase IV; 0301 basic medicine; replication; 0303 health sciences; [SDV]Life Sciences [q-bio]; Escherichia coli Proteins; DNA Helicases; 3. Good health; [SDV] Life Sciences [q-bio]; Rec A Recombinases; 03 medical and health sciences; DNA Gyrase; Mutation; Escherichia coli; bacteria; protein

ABSTRACT

The parE10 (Ts) mutation, which renders Escherichia coli thermosensitive for growth by inactivation of the essential E. coli topoisomerase topo IV, is lethal at all temperatures when PriA, the main replication restart protein, is absent. This lethality is suppressed by the activation of a PriA-independent replication restart pathway ( dnaC809 mutation). This result suggests that topo IV acts prior to full-chromosome replication completion.