16S rRNA Mutations That Confer Tetracycline Resistance inHelicobacter pyloriDecrease Drug Binding inEscherichia coliRibosomes

• DOI: 10.1128/jb.187.11.3708-3712.2005
• Publication Date: 2005-05-18T22:33:11Z
• Authors: Lisa Nonaka; Sean R. Connell; Diane E. Taylor
• Keywords: Ribosomal binding site

ABSTRACT Tetracycline resistance in clinical isolates of Helicobacter pylori has been associated with nucleotide substitutions at positions 965 to 967 the 16S rRNA. We constructed mutants which had different sequences rRNA gene present on a multicopy plasmid Escherichia coli strain TA527, all seven rrn genes were deleted. The MICs for tetracycline having single, double, or triple region that previously found highly resistant H. higher than mutant exhibiting wild-type sequence tetracycline-susceptible . MIC 965TTC967 substitution was 32 times E. 965AGA967 ribosomes extracted from tetracycline-resistant variant bound less this region. concentration ribosome 40% wild type. results study suggest binding primary site (Tet-1) is influenced by its patterns, form tetracycline.