Comparative Evaluation of the Nanosphere Verigene RV+ Assay and the Simplexa Flu A/B & RSV Kit for Detection of Influenza and Respiratory Syncytial Viruses

• DOI: 10.1128/jcm.02504-12
• Publication Date: 2012-11-15T04:33:50Z
• Authors: Kevin Alby; Elena B. Popowitch; Melissa B. Miller
• Keywords: 0301 basic medicine; 0303 health sciences; Clinical Laboratory Techniques; Respiratory Syncytial Virus Infections; Sensitivity and Specificity; Respiratory Syncytial Viruses; 3. Good health; Influenza B virus; 03 medical and health sciences; Influenza A virus; Nasopharynx; Virology; Influenza, Human; Humans; Prospective Studies; Retrospective Studies

ABSTRACT

Using retrospective ( n = 200) and prospective ( n = 150) nasopharyngeal specimens, we evaluated the Nanosphere Verigene RV+ and the Focus Diagnostics Simplexa Flu A/B & RSV tests. Overall, RV+ demonstrated sensitivities and specificities of 96.6% and 100% for influenza A virus, 100% and 99.7% for influenza B virus, and 100% and 100% for respiratory syncytial virus (RSV), while the Simplexa test sensitivities and specificities were 82.8 and 99.7%, 76.2 and 100%, and 94.6 and 100%, respectively.