Detection of Cytomegalovirus DNA in Human Specimens by LightCycler PCR

• DOI: 10.1128/jcm.38.11.4006-4009.2000
• Publication Date: 2019-12-31T17:50:36Z
• Authors: Lars Schaade; Peter Kockelkorn; Klaus Ritter; Michael Kleines
• Keywords: Betaherpesvirinae; Cytomegalovirus

ABSTRACT Detection of human cytomegalovirus (CMV) DNA in clinical specimens is considered a cornerstone the diagnosis CMV disease. The aim this study was to evaluate newly designed LightCycler-based quantitative PCR. Specimens origin ( n = 200) were tested using LightCycler PCR, COBAS AMPLICOR MONITOR (CACM) assay, and qualitative in-house PCR assay for presence DNA. Samples that reactive at least two three assays positive 95 [47.5%]), while samples nonreactive negative 105 [52.5%]). Using detected 91 DNA-positive (sensitivity, 95.8%; 95% confidence interval [CI], 89.6 98.8) 1 DNA-negative (specificity, 99%; CI, 94.8 99.8). Results load determination as assessed by both test systems correlated r 0.73; P < 0.0001; 0.61 0.81). undiluted containing high more accurate with than results obtained CACM test, which underestimated viral copy numbers. level sensitivity, specificity, accuracy, rapidity provided technology are favorable use system detection specimens.