Rapid Identification of Escherichia coli Pathotypes by Virulence Gene Detection with DNA Microarrays

• DOI: 10.1128/jcm.41.5.2113-2125.2003
• Publication Date: 2003-05-06T23:28:47Z
• Authors: Sadjia Bekal; Roland Brousseau; Luke Masson; Gabrielle Prefont...; John Fairbrother; Josée Harel
• Keywords: Amplicon; Virulence factor; Strain (injury)

ABSTRACT One approach to the accurate determination of pathogenic potential (pathotype) isolated Escherichia coli strains would be through a complete assessment each strain for presence all known E. virulence factors. To accomplish this, an factor DNA microarray composed 105 PCR amplicons printed on glass slides and arranged in eight subarrays corresponding different pathotypes was developed. Fluorescently labeled genomic DNAs from representing were initially hybridized gene microarrays both chip optimization validation. Hybridization pattern analysis with clinical isolates permitted rapid their attributes group which they belonged. Virulence factors belonging two detected one human isolate (strain H87-5406). The also tested its ability distinguish among phylogenetic groups genes by using probes derived attaching-and-effacing locus ( espA , espB tir ). After hybridization these probes, we able harboring sequences closely related enterohemorrhagic (EDL933), enteropathogenic (E2348/69), or animal (RDEC-1). Our results show that is powerful tool diagnosis-based studies concept useful quantitation subtyping. Additionally, multitude present should greatly facilitate detection acquired horizontal transfer identification emerging pathotypes.