Role of the membrane-associated accessory protein (MAAP) in adeno-associated virus (AAV) infection

• DOI: 10.1128/jvi.00633-24
• Publication Date: 2024-05-22T13:01:39Z
• Authors: Cagla Aksu Kuz; Kang Ning; Siyuan Hao; Fang Cheng; Jianming Qiu
• Keywords: Adeno-associated virus

Adeno-associated viruses (AAVs) package a single-stranded (ss) DNA genome of 4.7 kb in their capsid ~20 nm diameter. AAV replication requires co-infection helper virus, such as adenovirus. During the optimization recombinant production, small viral nonstructural protein, membrane-associated accessory protein (MAAP), was identified. However, function MAAP context infection remains unknown. Here, we investigated expression strategy and during both AAV2 AAV5 human embryonic kidney (HEK)293 cells. We found that MAAP2 MAAP5 are expressed from gene (