Discovery of Novel Thrips Vector Proteins That Bind to the Viral Attachment Protein of the Plant Bunyavirus Tomato Spotted Wilt Virus

• DOI: 10.1128/jvi.00699-19
• Publication Date: 2019-08-12T12:29:06Z
• Authors: Ismael E. Badillo...; Yuting Chen; Kathleen M. Martin; Dorith Rotenberg; Anna E. Whitfield
• Keywords: Tospovirus; Viral protein; Wilt disease

Thrips-transmitted viruses cause devastating losses to numerous food crops worldwide. For negative-sense RNA that infect plants, the arthropod serves as a host well by supporting virus replication in specific tissues and organs of vector. The goal this work was identify thrips proteins bind directly viral attachment protein thus may play role infection cycle insect. Using model plant bunyavirus tomato spotted wilt (TSWV), most efficient vector, we identified validated six TSWV-interacting from Frankliniella occidentalis first-instar larvae. Two proteins, an endocuticle structural glycoprotein cyclophilin, were able interact with TSWV protein, G N , insect cells. -interacting provide new targets for disrupting disease vector could be putative determinants competence.