Identification of host dependency factors involved in SARS-CoV-2 replication organelle formation through proteomics and ultrastructural analysis

• DOI: 10.1128/jvi.00878-23
• Publication Date: 2023-10-31T13:01:10Z
• Authors: Felix Pahmeier; Teresa-Maria Lavacca; Sarah Goellner; Christopher J. Ne...; Vibhu Prasad; Berati Cerikan; Sreejith Rajasekh...; Giulia Mizzon; Uta Haselmann; Charlotta Funaya; Pietro Scaturro; Mirko Cortese; Ralf Bartenschlager
• Keywords: Endomembrane system; Organelle

Remodeling of the cellular endomembrane system by viruses allows for efficient and coordinated replication viral genome in distinct subcellular compartments termed organelles. As a critical step life cycle, organelle formation is an attractive target therapeutic intervention, but factors central to this process are only partially understood. In study, we corroborate that two proteins, nsp3 nsp4, major drivers membrane remodeling SARS-CoV-2 infection. We further report number host cell interacting with these proteins supporting some them contributing organelle.