The African Swine Fever Virus DP71L Protein Recruits the Protein Phosphatase 1 Catalytic Subunit To Dephosphorylate eIF2α and Inhibits CHOP Induction but Is Dispensable for These Activities during Virus Infection

• DOI: 10.1128/jvi.01027-10
• Publication Date: 2010-08-12T02:28:22Z
• Authors: Fuquan Zhang; Alice Moon; Kay Childs; Stephen Goodbourn; Linda K. Dixon
• Keywords: Protein phosphatase 1; Dephosphorylation; EIF4EBP1

ABSTRACT The African swine fever virus (ASFV) DP71L protein is present in all isolates as either a short form of 70 to 72 amino acids or long about 184 acids, and both these share sequence similarity the C-terminal domain herpes simplex ICP34.5 cellular GADD34. In study we expressed different mammalian cells demonstrated that causes dephosphorylation eukaryotic translation initiation factor 2 alpha (eIF2α) resting during chemical-induced endoplasmic reticulum stress acts enhance expression cotransfected reporter genes. We showed binds three isoforms (α, β, γ) phosphatase 1 catalytic subunit (PP1c) by recruiting PP1c eIF2α. also inhibits induction ATF4 its downstream target, CHOP. investigated eIF2α phosphorylation status CHOP porcine macrophages infected two ASFV field isolates, Malawi Lil20/1 Benin 97/1, deletion mutants, MalawiΔNL E70ΔNL. Our results gene did not cause an increase level CHOP, indicating only required control infection. therefore hypothesize has other mechanisms prevent subsequent synthesis inhibition.