Recombinant VP4 of Human Rhinovirus Induces Permeability in Model Membranes

• DOI: 10.1128/jvi.01070-07
• Publication Date: 2008-02-07T01:45:27Z
• Authors: Matthew P. Davis; Graham Bottley; Lucy P. Beales; Richard A. Killin...; David J. Rowlands; Tobias J. Tuthill
• Keywords: 0303 health sciences; 03 medical and health sciences; Membranes; Rhinovirus; Liposomes; Humans; Capsid Proteins; Virus Internalization; Permeability; Recombinant Proteins; Protein Binding; 3. Good health

ABSTRACT In common with all nonenveloped viruses, the mechanism of picornavirus membrane penetration during cell entry is poorly understood. The small, myristylated capsid protein VP4 has been implicated in this process. Here we show that recombinant VP4 of human rhinovirus 16 has the ability to associate with and induce membrane permeability in otherwise intact liposomes. This provides further evidence that VP4 plays a key role in picornavirus cell entry.